Regulatory

Part:BBa_K1111001:Design

Designed by: Luisa Spisak   Group: iGEM13_EPF_Lausanne   (2013-09-18)
Revision as of 12:47, 27 September 2013 by GigiGoesGugu (Talk | contribs) (→‎References)


Hya promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 184
    Illegal AgeI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

took 500bp upstream from starting codon


Source

Escherichia Coli, K-12, Substrain MG1655

References

Journal of Bacteriology
Response of hya Expression to External pH in Escherichia coli
Paul W. King and Alan E. Przybyla
J.Bacteriol. 1990
http://jb.asm.org/content/181/17/5250.full

Journal of Bacteriology
Mutational analysis and charecterization of the Escherichia coli hya operin, which encodes [NiFe] hydrogenase1
N K Menon, J Robbins, J C Wendt, K T Shanmuagam and A E Przybyla
J. Bacteriol 1991