Device

Part:BBa_K1065101:Design

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-06-25)
Revision as of 12:44, 27 September 2013 by Cate (Talk | contribs) (→‎Design Notes)


AraCpBAD + BSMT1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1893
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1434
    Illegal SapI site found at 961


Design Notes

This BioBrick was cloned starting from BBa_K1065100 and added BBa_K731201 following the standard assembly protocol.

Source

AraC-pBAD was extracted from E. coli. BSMT1 was extracted from Petunia x hybrida.

References

1. Florence Negre at al., Novel S-adenosyl-l-methionine:salicylic acid carboxyl methyltransferase, an enzyme responsible for biosynthesis of methyl salicylate and methyl benzoate, is not involved in floral scent production in snapdragon flowers, Archives of Biochemistry and Biophysics, Volume 406, Issue 2, 15 October 2002, Pages 261–270.