Coding

Part:BBa_K1104100

Designed by: Yi-Hao Huang   Group: iGEM13_NYMU-Taipei   (2013-09-17)
Revision as of 12:51, 4 October 2013 by HeidiChen (Talk | contribs)

mngB - alpha-mannosidase

mngB, a passage of gene in Escherichia coli K-12 substr MG1655, can produce alpha-mannosidase. It is an enzyme that turns 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate, which is the reverse reaction of the post-translation (glycosylation) on polar filaments of Fungi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 716
    Illegal BglII site found at 992
    Illegal BglII site found at 2139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 235
    Illegal AgeI site found at 2242
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None