Part:BBa_K1228008

38aa with T1 terminator and Constitutive promoter veg

Human Llysozyme (hLZ), the major protein in breast milk, exhibits microbicidal activity to various degrees against several bacterial strains. This Generator can secrete the HLH peptide(38 aminoacid peptides) that is a part of Human Lysozyme in B.subtilis. The HLH peptide and its N-terminal helix (H1) were the most potent bactericidal to bacteria. Evidence shows that HLH peptide and its N-terminal helix (H1) kill bacteria by crossing the outer membrane of Gram-negative bacteria via self-promoted uptake and is able to dissipate the membrane-potential-dependent respiration of Gram-positive bacteria.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Experiment

In order to distinguish whether the B. Subtilis express our objective protein or not,we used the method of in-vitro bacterial inhibition zone to observe the anti-bacterial effect.We added 100 ul B. Subtilis liquid medium into the hole. The inhibition zone results showed that gram-positive bacterium was sensitive to 38aa.

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Reference

1.Hisham R. Ibrahim,* Kenta Imazato, and Hajime Ono. Human Lysozyme Possesses Novel Antimicrobial Peptides within Its N-terminal Domain that Target Bacterial Respiration. J Agric Food Chem. 2011 Sep 28;59(18):10336-45. doi: 10.1021/jf2020396. Epub 2011 Sep 2.