Regulatory
PphoA

Part:BBa_K1139200:Design

Designed by: Sara Ogino   Group: iGEM13_Tokyo_Tech   (2013-09-24)
Revision as of 11:35, 27 September 2013 by Tryalnigro (Talk | contribs) (→‎Design Notes)


phoA promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

phoA promoter region of E. coli was amplified from MG1655 genomic DNA by PCR.
Upstream primer (5’-acgtgaattcgcggccgcttctagagaaagttaatcttttcaacagctgtcataaag-3’)
Downstream primer (5’-ccgctactagtaaatacattaaaaaataaaaacaaagcgactataagtctc-3’)
[http://regulondb.ccg.unam.mx/operon?term=ECK120014865&format=jsp&organism=ECK12&type=operon#phoAp details]

References

1. M. Dollard et al. “Whole-cell bacterial sensors for the monitoring of phosphate availability” Journal of Microbiological Methods (2003)
2. H. Shinagawa et al. “Regulation of the pho Regulon in Escherichia coli K-12” J. Mol. Biol. (1983)
3. Y. Hsieh et al. “Global regulation by the seven-component Pi signaling system” Current Opinion in Microbiology (2010)
4. F. Neidhardt et al. “Culture Medium for Enterobacteria” Journal of Bacteriology (1974)
5. CCG/UNAM. Regulon DB. http://regulondb.ccg.unam.mx/index.jsp (accessed 2013-09-25)
6. D. R. Hoagland and D. I. Arnon (1950), The water-culture method for growing plants without soil. California agrigultual experiment station circular, 347, 1-32