Composite

Part:BBa_K1139022:Design

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-09-24)
Revision as of 10:05, 27 September 2013 by 382069 (Talk | contribs) (→‎Source)

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Promoterless-M13-Plac-GFP on pSB3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6026
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7287


Design Notes

sequence confrirmed

Source

The plasmid construction is as follows. First, from M13mp18 phage vector, we amplified the sequence that includes from g2p RBS to M13 origin (with prefix and suffix sequence), by PCR. Second, we inserted the amplified sequence to pSB3K3 backbone.

References

1. Atsushi Higashitani. Nahoko Higashitani. Kensuke Horiuchi, DNA Replication in Filamentous Bacteriophage, Protein, Nucleic Acid and Enzyme(1994), vol39:2189-2197.
2. Kensuke Horiuchi, Origin of DNA replication of filamentous coliphages, Jpn. J. Genet(1990), vol65:225-241.
3. ME Ortiz. D Endy., Engineered cell-cell communication via DNA messaging, J. Biol. Engineering(2012), 6-16.
4. A Goñi-Moreno, M Amos, F de la Cruz, Multicellular Computing Using Conjugation for Wiring, PLOS ONE(2013), Vol8.:Issue6:e65986.
5. M Shintani. N Fukushima. M Tezuka. H Yamane. H Nojiri, Conjugative transfer of the IncP-7 carbazole degradative plasmid, pCAR1, in river water samples, Biotechnol Lett (2008). vol30:117-122
6.H Nojiri Structural and Molecular Genetic Analyses of the Bacterial Carbazole Degradation System Biosci, Biotechnol, Biochem, 76(1), 1-18, 2012