Composite

Part:BBa_K1036003:Design

Designed by: Xin Guo, Xi Xi   Group: iGEM13_XMU-China   (2013-08-27)
Revision as of 07:00, 27 September 2013 by Tina Zhang (Talk | contribs) (→‎Source)

lux pL controlled luxR with lux pR controlled gfp (LVA-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3909
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1828
    Illegal BsaI.rc site found at 2086
    Illegal BsaI.rc site found at 3189


Design Notes

This part contains two subparts: BBa_F2621 (located in 2012-Plate 2-21F) and BBa_K1036002.
BBa_F2621 digested by EcoR & SpeI and BBa_K1036002 digested by EcoR & XbaI were ligated together by T4 ligase.

Source

We used the biobrick BBa_F2621 (located in 2012-Plate 2-21F)

References

Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)