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Part:BBa_K1189006

Designed by: Sharon Feng and Julie Osgood   Group: iGEM13_Calgary   (2013-09-16)
Revision as of 04:10, 27 September 2013 by Sharonfeng1 (Talk | contribs)

TALEA and TALEB Target

Using the target sequences created for both TALA (BBa_K782004) and TALB (BBa_K782006), we assembled this target sequence construct to be used in our proof of concept system. Because TALE B was discovered to have a mutation, we have two versions of this construct. One has the original TALE B target sequence we created assuming there was no mutation. This is the new target sequence.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 213
  • 1000
    COMPATIBLE WITH RFC[1000]


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