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Part:BBa_K1189005:Design
Designed by: Sharon Feng Group: iGEM13_Calgary (2013-09-16)
Revision as of 22:51, 27 September 2013 by Sharonfeng1 (Talk | contribs)
TALEB Target ([B])
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 213
Illegal AgeI site found at 325 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed primers that would produce these TALE B target sequence in a KAPA PCR reaction. Our target sequences were then ligated into RFP generator, in a pSB1C3 backbone. We expected a sequence of AGCAATGGG in the repeat variable di-residue of the second repeat of TALB, however, the sequence was actually TCCCACGAC. This meant that the required target sequence at this position was a C, and not a T, as the parts registry web page indicates. Therefore, this part has the updated TALB target sequence with C instead of the T.
Source
The target sequence was amplified with primers from the genome of E. coli using polymerase chain reaction.