Part:BBa_K1074007

Pctc+RBS+amilCP

Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). amilCP(BBa_K592009), blue chromoprotein,naturally exhibits strong color when expressed. when bacterial grow to the post-log phase, nutrient and space stress arise,Promoter ctc should be activited. We assemble this gene circuit just to expect a visual signal when B.subtilis grow to the post-log phase.

Reference

Benson, A.K. and W.G. Haldenwang, Characterization of a Regulatory Network That Controls Sigma-B Expression in Bacillus-Subtilis. Journal of Bacteriology, 1992. 174(3): p. 749-757. Boylan, S.A., et al., Activation of Bacillus-Subtilis Transcription Factor Sigma(B) by a Regulatory Regulatory Pathway Responsive to Stationary-Phase Signals. Journal of Bacteriology, 1992. 174(11): p. 3695-3706. Boylan, S.A., et al., Stress-Induced Activation of the Sigma(B) Transcription Factor of Bacillus-Subtilis. Journal of Bacteriology, 1993. 175(24): p. 7931-7937. Brody, M.S., K. Vijay, and C.W. Price, Catalytic function of an alpha/beta hydrolase is required for energy stress activation of the sigma(B) transcription factor in Bacillus subtilis. Journal of Bacteriology, 2001. 183(21): p. 6422-6428. Delumeau, O., R.J. Lewis, and M.D. Yudkin, Protein-protein interactions that regulate the energy stress activation of sigma(B) in Bacillus subtilis. Journal of Bacteriology, 2002. 184(20): p. 5583-5589.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 78
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 78
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 78
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 78
  
• 1000
  COMPATIBLE WITH RFC[1000]