RNA
Part:BBa_K1100061
Designed by: Hao-tian Guo Group: iGEM13_Fudan (2013-09-13)
RNA-OUT S1
It is the wild type RNA-OUT of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT.It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.
References
Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
biology | |
direction | Forward |
negative_regulators | 1 |
origin | Escherichia coli |
positive_regulators |