Composite

Part:BBa_K1017101

Designed by: Wen-Hui Cheng   Group: iGEM13_NCTU_Formosa   (2013-09-12)
Revision as of 15:39, 26 September 2013 by AggieTsai (Talk | contribs)

Pred ( PompC + B0032 + LacI + J61048 + Plac)

In general, the red light sensing promoter, OmpC promoter, turns on in the dark and turns off under red light with the present of Cph8.

Nctu formosa cph8mechnism.jpg



But we want to construct a more intuitive light regulating th system at can turn on under red light and turn off in the dark. So we created a new biobrick named red promoter by adding a lac operon to inverse the function of OmpC promoter.

Pred, including PompC,RBS(B0032), LacI, ter(J61048), and Plac, can be activated when exposed to red light. OmpR, the transcription factor of PompC, is active when being phosphorylated in dark and inactive under red light. In the presence of red light, the non phosphorylated OmpR cannot turn on PompC. Thus, Plac won't be repressed by the negative regulator, LacI. So the whole Pred can express genes downstream Plac. On the other hand, if there is no red light, the phosphorylated OmpR then turn on PompC, causing LacI to be expressed and repress Plac to translate the genes downstream.

Nctu formosa pred.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1282
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1296
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None