Regulatory
pRhl + RBS

Part:BBa_K1073001

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)
Revision as of 10:51, 28 September 2013 by TUNKI (Talk | contribs) (→‎Usage and Biology)

Inducible promoter of the rhl quorum sensing system with RBS

This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of P. aeruginosa which is activated by RhlR in combination with N-buturyl homoserine lactone (N-buturyl-HSL) with an RBS.


Usage and Biology

The device can be cloned in front of a desired gene of interest in order to induce its expression by N-buturyl-HSL. For further information seeThe inducible Promotor from Pseudomonas aeruginosa is activated by RhlR complexed with the specific autoinducer N-buturyl-HSL produced by RhlI. The induction of the promoter in P. aeruginosa leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. 

BBa_R0071.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None