Part:BBa_K1017202

Regulation RBS-2

sRNA are small (50-250 nucleotide) non-coding RNA molecules produced by bacteria; they are highly structured and contain several stem-loops.sRNAs interact with the targeted mRNAs by imperfect base pairing, so sRNAs can bind to mRNA targets occludes the Shine-Dalgarno sequence thus prevents the ribosome from binding to the initiation condon, so the translation would be repressed.In our project we use the artificial sRNA(BBa_K1017404[1]), which needed to complementary with the SD sequence in the RBS, targets specifically to the desired genes. Therefore, we also need to design a RBS which only would be bound with our artificial sRNA. Here we call this RBS as rRBS.

We designed the rRBS by employing the sRNA targeting region from ompF so that the sRNA would only complementary with ompF but not the others in the E.coli we use. We also make the AUG codon sufficiently apart from the SD sequence for ribosome binding. Therefore, other iGEM teams can use this sRNA regulation system in there project by adding this RBS to the upstream of any desired gene, the gene can be regulated by sRNA.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]