Coding

Part:BBa_K1014000:Design

Designed by: Xiangmiao Zeng   Group: iGEM13_HIT-Harbin   (2013-08-28)
Revision as of 06:06, 26 September 2013 by Sema1688 (Talk | contribs) (→‎References)

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HrpS Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
    Illegal AgeI site found at 163
    Illegal AgeI site found at 298
    Illegal AgeI site found at 456
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TAATAA double stop codon added.

Codon optimization for E.coli was done using [http://www.jcat.de/ JCat] an online Codon Adaptation Tool which was published in NAR (Nucleic Acids Research).

Codon optimization results was present on [http://2013.igem.org/Team:HIT-Harbin/Parts our wiki]

Source

[http://www.ncbi.nlm.nih.gov/nuccore/NC_014500.1?report=genbank&from=2646451&to=2647434&strand=true GeneBank NC_014500.1]

BBa_M39011

References

[http://jb.asm.org/content/193/8/2076.short J. Bacteriol. 193 (8), 2076-2077 (2011)]

[http://nar.oxfordjournals.org/content/33/suppl_2/W526.short Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host]