Composite
Part:BBa_K1166002
Designed by: Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero Group: iGEM13_TecMonterrey (2013-09-17)
Revision as of 21:33, 25 September 2013 by LuisFernandoCG (Talk | contribs)
This is a device that allows a protein to be secreted by means of the alpha-hemolysin secretion system in E. coli.
It’s designed so that the only thing that you have to do is to assemble your protein part in the device via the biofusion standard (BBF RFC 23), (Cut the device with EcoRI and XbaI, cut your protein part with EcoRI and SpeI, mix & ligate). This procedure will leave your part in-frame with a signal peptide.
Note: It’s important that your part doesn’t contain a stop codon nor a terminator since your protein will be fused at the C-terminus.
[edit]
Categories
Parameters
| None |