DNA

Part:BBa_K1031011

Designed by: He Shuaixin   Group: iGEM13_Peking   (2013-09-09)
Revision as of 18:30, 25 September 2013 by Psyche (Talk | contribs)

B0034-RiboJ-φR73



Component and Characterization

K1031011 consists of RBS B0034[1], ribozyme RiboJ and transcription factor φR73 delta. φR73 functions as an activator of hybrid promoter[2]. It is the activation branch of the Band-pass Filter circuit (Fig 1).

Fig 1 Activation circuit of Band-pass Filter. The green oval represents RBS B0034. Ribozyme RiboJ is marked in dark blue. φR73 coding sequence is shown as square with dark red. φR73 activates hybrid promoter, which is marked with red and blue, via binding upstream activating sequence. Then the expression of sfGFP is enhanced.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Data shown

Previous work done by Dr. Lou Chunbo showed φR73 had obvious activating effect to downstream promoter. Molecular cloning which connected φR73 with pSal promoter enables the circuit to response to salicylic acid (Fig 2).

Fig 2 Induction ratio of activation circuit when exposed to inducers. Horizontal axis represents salicylic acid at different concentration. Vertical axis stands for induction ratio calculated from fluorescence intensity.


[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/bacteriophage
//function/regulation/transcriptional
Parameters
positive_regulators