Part:BBa_K1031015

NahR-Psal-B0032-RiboJ-CI

Components and Characterization

K1031015 consists of NahR[1], Psal promoter, RBS B0032[2] and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter[3]. It is the repression branch of the Band-pass Filter circuit (Fig 1). In order to fine-tune the expression level of transcriptional repressor, we adjusted the RBS (Ribosome Binding Site) intensity in front of CƖ. K1031015 adopts RBS B0032.

Fig 1 Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once Psal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP. The RBS B0032 is shown in light green, regulating the expression level of CƖ protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 576
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 408
  
• 1000
  COMPATIBLE WITH RFC[1000]

Data shown

Fig 2 Induction ratio of repression circuit adopting RBS B0032 when exposed to inducers. Horizontal axis represents salicylic acid at different concentration. Vertical axis stands for induction ratio calculated from fluorescence intensity.