Composite
M13 genes

Part:BBa_K1139019

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-09-01)
Revision as of 18:29, 26 September 2013 by Keso57 (Talk | contribs)

Promoterless-M13 on pSB3

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector, amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter on the upstream of g2p is deleted. The capsid is formed only when g2p is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6027
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aPromoterless-M13 on pSB3