RNA
MicC scaff

Part:BBa_K1124005:Design

Designed by: Yuta Otsuka   Group: iGEM13_UT-Tokyo   (2013-09-11)
Revision as of 04:06, 24 September 2013 by YutaOtsuka (Talk | contribs) (→‎References)

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MicC sRNA scaffold (w/o antisense)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

MicC scadffold sequence is 79bp. (The 85bp sequence in [1] contains 6bp of Xho1 restriction site.)

Source

Artificial synthesis (PCR using primers shown on our wiki (currently under construction))

References

[1] Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013). Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature biotechnology.