Generator

Part:BBa_K1073013

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)
Revision as of 18:46, 26 September 2013 by Laura S (Talk | contribs)

Inducible ampicillin resistance cassette (regulated by LasR + N-3-oxododecanoyl-HSL)

This device is a combination of BBa_K1073003 and BBa_K1073000 and mediates a inducible ampicillin resistance. It includes the inducible promoter of the las quorum sensing system of Pseudomonas aeruginosa followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.


Usage and Biology

The promoter is activated in the presence of LasR in combination with the autoinducer molecule N-3-oxododecanoyl-homoserine lactone produced by LasI. The expression of the beta-lactamase can therefore be induced and tuned by supplying LasR and N-3-oxododecanoyl-HSL.

Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073034.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None