Coding

Part:BBa_K1067003

Designed by: Kristian Davidsen and Helen Cook   Group: iGEM13_DTU-Denmark   (2013-09-19)
Revision as of 18:11, 23 September 2013 by KrDa (Talk | contribs)

Ammonium monooxygenase from Nitrosomonas europaea

Ammonia monooxygenase (AMO) from Nitrosomonas europaea converts ammonia to hydroxylamine; NH3 → NH2OH

The whole operon containing amoA1, amoB1, amoC1 [http://biocyc.org/NEUR228410/NEW-IMAGE?type=OPERON-IN-CHROM-BROWSER&object=TUJNO-524 Biocyc] was extracted by colony PCR on a N. europaea culture.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 263
    Illegal BamHI site found at 306
    Illegal BamHI site found at 2510
    Illegal BamHI site found at 2822
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2028
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//function/biosynthesis
Parameters
protein