Coding
"Eye 1"
Part:BBa_K1080000:Design
Designed by: Macquarie University Group: iGEM13_Macquarie_Australia (2013-09-22)
ChlI1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 231
Illegal BglII site found at 887
Illegal BglII site found at 1082
Illegal BamHI site found at 279 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence
Source
Chlamydomonas reinhardtii