Part:BBa_K1080004:Design
ChlM
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
ChlM Clone: DNA sequence from translation start site: Regions in BOLD are the sequence of the leader region in the pET100 plasmid. Translated the DNA sequence into a protein sequence using "Translate" at http://au.expasy.org/tools Then used the translated protein sequence to analyse the protein using "ProtParam" at http://au.expasy.org/tools
No Biobrick sites: PstI, XbaI, SpeI or EcoRI
ATG CGG GGT TCT CAT CAT CAT CAT CAT CAT GGT ATG GCT AGC ATG ACT GGT GGA
CAG CAA ATG GGT CGG GAT CTG TAC GAC GAT GAC GAT AAG GAT CAT CCC TTC ACC
ATGGCTTCTGAGATTGCTCAAACTGCCGATGTTGGCTCGCTGACCTTCGCGGTTGGCGGA
GTGGGCGCTGTTGTGGGCCTGGGCGCCCTGCTTGTCGCTACTGACCCTCAGAAGCGCCGC
TCGGAGCAGATGAAGTCATTCGACGGCGATGAGAAGGAGGCGGTGAAGGACTACTTCAAC
ACTGCCGGCTTCGAGCGCTGGCGCAAGATCTACGGCGAGACGGACGAGGTGAACAAGGTG
CAGCTGGACATCCGCACTGGCCACGCGCAGACGGTGGACAAGGTGCTGCGCTGGGTGGAC
GAGGAGGGCAGCGTGCAGGGCATCACCGTGGCGGACTGCGGCTGCGGGACAGGCAGCCTG
GCCATCCCGCTGGCGCTGCGCGGCGCCGCCGTCAGCGCCTCCGACATCTCCGCCGCCATG
GCGTCTGAGGCGGAGCAGCGCTACCAGCAGGCGGTGGCGGCCGGGCAGGGCAAGGCGCCC
AAGGTGGCGCCCAAGTTCGAGGCGCTGGACCTGGAGTCCGTCAAGGGCAAGTACGACACG
GTCACTTGCCTGGACGTGATGATTCACTACCCCCAGGACAAGGTGGACGCCATGATCACG
CACCTGGCGGGTCTGTCCGACCGCCGCCTCATCATCTCGTTCGCACCCAAGACGCTGTCG
TACAGCATCCTCAAGCGCATTGGCGAGCTGTTCCCCGGCCCCAGCAAGGCCACCCGCGCC
TACCTGCACCGTGAGGAGGACGTGGAGGCTGCCCTCAAGCGCGCCGGCTTCAAGGTCACC
AAGCGTGAGATGACCGCCACCAGCTTCTACTTCTCGCGCCTGCTGGAGGCCATCCGCGAG
TAATCTCGCAAACGTTCTCGTGTAAATAGCGTAGCAGTGCAGGACTGGTGTAAGGACTGA
TGGACTCGGACCGGCGCTGCGGCCGGGTATGAGGGCATGGGCGTGGGCGCTGCTAGGGTG
CTACGGTGCAGCGCGGTTTTGACGGGCTATAGACATTTATGTGACTGAGTGACGGGCGGA
TAGCCAAAACGATTGTGTGAACG
Amino acid sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDHPFTMASE IAQTADVGSL TFAVGGVGAV
VGLGALLVAT DPQKRRSEQM KSFDGDEKEA VKDYFNTAGF ERWRKIYGET DEVNKVQLDI
RTGHAQTVDK VLRWVDEEGS VQGITVADCG CGTGSLAIPL ALRGAAVSAS DISAAMASEA
EQRYQQAVAA GQGKAPKVAP KFEALDLESV KGKYDTVTCL DVMIHYPQDK VDAMITHLAG
LSDRRLIISF APKTLSYSIL KRIGELFPGP SKATRAYLHR EEDVEAALKR AGFKVTKREM
TATSFYFSRL LEAIRE
Number of amino acids: 316
Molecular weight: 34495.0
Theoretical pI: 6.14
Amino acid composition:
Ala (A) 37 11.7% Arg (R) 19 6.0% Asn (N) 2 0.6% Asp (D) 24 7.6% Cys (C) 3 0.9% Gln (Q) 13 4.1% Glu (E) 20 6.3% Gly (G) 28 8.9% His (H) 11 3.5% Ile (I) 13 4.1% Leu (L) 23 7.3% Lys (K) 20 6.3% Met (M) 10 3.2% Phe (F) 11 3.5% Pro (P) 9 2.8% Ser (S) 20 6.3% Thr (T) 19 6.0% Trp (W) 2 0.6% Tyr (Y) 9 2.8% Val (V) 23 7.3%
Asx (B) 0 0.0% Glx (Z) 0 0.0% Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 44 Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1509 Hydrogen H 2396 Nitrogen N 432 Oxygen O 468 Sulfur S 13
Formula: C1509H2396N432O468S13 Total number of atoms: 4818
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm.
Ext. coefficient 24535 Abs 0.1% (=1 g/l) 0.711, assuming ALL Cys residues appear as half cystines
Ext. coefficient 24410 Abs 0.1% (=1 g/l) 0.708, assuming NO Cys residues appear as half cystines
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is:
30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.76 This classifies the protein as stable.
Aliphatic index: 77.25
Grand average of hydropathicity (GRAVY): -0.339
Source
Chlamydomonas reinhardtii