Regulatory
FUS1 pr

Part:BBa_K1154001:Design

Designed by: Adam Nighswander, Tanner Reeb and Bianca Maled   Group: iGEM13_RHIT   (2013-09-17)
Revision as of 00:53, 21 September 2013 by RicRHIT (Talk | contribs) (→‎Design Notes)

Saccharomyces cerevisiae FUS1 promoter (induced by mating pheromone)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is from Saccharomyces cerevisiae Reference Strain S288C. It is very similar to the FUS1 promoter well characterized by the 2012 TU-Delft iGEM Team (see Part:BBa_K775004). However, the sequences do differ:

File:Parts.igem.org/wiki/images/c/c8/Align BBa K775004 BBa K1154001.JPG

Source

Sequence is from the genome of Saccharomyces cerevisiae Reference Strain S288C chromosome III coordinates 71603 to 71803, in the promoter region of the FUS1/YCL027W coding sequence ([http://www.yeastgenome.org Saccharomyces Genome Database]).

References

McCaffrey G, Clay FJ, Kelsay K, and Sprague GF Jr (1987) Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7 (8), 2680-2690.