Regulatory

Part:BBa_K1153000

Designed by: Kara Stubbs, David Hanly, Laura Carman, Sarah Dowie, Michael Coghlan, Rathaven Gunaratnarajah.   Group: iGEM13_Kent   (2013-09-20)
Revision as of 22:26, 16 October 2016 by Aasli (Talk | contribs)

NorV promoter. NOx detector.

Our Biobrick has been designed to enable the detection of NO using the norV gene promoter, cloned from E.coli K12 TOP10 cells.

In the presence of NOx it will promote the downstream genes in the plasmid, producing a protein in response to the environmental stimulus.

Contribution Group: ETH Zurich 2016 Author: Asli Azizoglu Summary: We produced and characterised the norV promoter, and sent it to the registry as a biobrick.

Promoter norV (PnorV) is the native promoter controlling the nitric oxide reduction operon (norRVW) in E. Coli. [1] It's transcriptional regulator, NorR, can sense nitric oxide and activate gene expression.


References: [1] Gardner, A. M. "Regulation Of The Nitric Oxide Reduction Operon (Norrvw) In Escherichia Coli. ROLE OF Norr AND Sigma 54 IN THE NITRIC OXIDE STRESS RESPONSE". Journal of Biological Chemistry 278.12 (2003): 10081-10086.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None