Composite

Part:BBa_K1154000:Design

Designed by: Adam Nighswander, Tanner Reeb and Bianca Maled   Group: iGEM13_RHIT   (2013-09-16)
Revision as of 12:00, 20 September 2013 by RicRHIT (Talk | contribs) (→‎Design Notes)

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Mating pheromone-induced IGPD and constitutive LDH expression in Yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 763
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was designed for high codon bias in Saccharomyces cerevisiae and conformity with current Registry standards. Its component sequences can be found in the Registry (see Source).

Source

This sequence was commercially synthesized. Component sequences can be found in the Registry: Yeast FUS1 promoter (BBa_K1154001); Yeast TEF2 promoter (BBa_K165037); Yeast ADH1 terminator (BBa_J63002); Yeast imidazoleglycerol-phosphate dehydratase (IGPD) coding sequence (BBa_K1154002); Human lactate dehydrogenase A (LDHA) coding sequence (BBa_K1154003). However, assembling these sequences using the Composite Parts Tool will not produce a sequence identical to the one submitted here.

References