Reporter

Part:BBa_K1100006:Design

Designed by: Hao-tian Guo   Group: iGEM13_Fudan   (2013-09-13)
Revision as of 10:19, 20 September 2013 by Shuqidu (Talk | contribs) (→‎Source)

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optimized sfGFP with Csy4 insulator loci


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A optimized sfGFP with a Csy4 loci, can be used as a powerful reporter. You can get this part from BBa_K1100015 and other parts we have submitted.

Source

Insulator: Csy4 loci via PCR, B0034 via PCR, sfGFP from Ding Yu's Lab at Fudan University

References

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006.