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ccdB_Ind

Part:BBa_K1152007:Experience

Designed by: Joshua Sachs   Group: iGEM13_Heidelberg   (2013-09-20)
Revision as of 17:50, 2 October 2013 by Philipp.walch (Talk | contribs) (→‎Applications of BBa_K1152007)


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Applications of BBa_K1152007

pJS01 was amplified out of four fragments via Gibson Cloning: ccdB, the C-domain of TycC2 and the indC-gene, as well as a backbone. We verified the genotype by colony-PCR, restriction digest with various restriction enzymes, among them EcoRI and PstI, and sequencing of the assembly overhang regions. One part of the standard, we propose the possibility to tag Non-Ribosomal Peptides with Indigoidine. Using this helper-construct eased our work in bringing the proof of principle for the functionality of the Indigoidine-tag, as by replacing the ccdB with the NRP of interest, relegation-background is significantly decreased. This construct stands as representative of the various fusion-peptides with Indigoidine-tag that we synthesized during our project.

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