Coding
Part:BBa_K1076003:Design
Designed by: Carlos Gustavo Nunes Group: iGEM13_Manaus_Amazonas-Brazil (2013-09-13)
FadD, Long-chain-fatty-acid-CoA ligase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1477
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1631
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1210
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 163
Design Notes
We chosed this ORF gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3. Further subclonning and expression should be done within pSB3C5. Should also be assembled with promoter, RBS and terminator.
Source
From Shewanella putrefaciens genomic sequence