Device

Part:BBa_K1088027:Experience

Designed by: Patrick Rosendahl Andreassen   Group: iGEM13_SDU-Denmark   (2013-09-19)
Revision as of 15:16, 4 October 2013 by P.R.A (Talk | contribs) (→‎User Reviews)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1088027

User Reviews

UNIQ5b68e81d2a0c8667-partinfo-00000000-QINU UNIQ5b68e81d2a0c8667-partinfo-00000001-QINU

•••

Patrick Rosendahl Andreassen

SDU2013_Characterization_Dxs_2.png

Growth curve of dxs devices. Cells were started at OD600=0.005 and incubated at 37oC and 180 rpm. 2 triplicates of MG1655 carrying no vector (WT), empty pSB1C3 vector (pSB1C3), pSB1C3-Plac-dxs(B.subtilis) (BBa_K1088011) (No LacI), pSB1C3-Pcon-lacI-Plac-dxs(B.subtilis) (BBa_K1088027) (LacI(N)), or pSB1C3-Pcon-lacI:LVA-Plac-dxs(B.subtilis) (BBa_K1088013) (LacI(LVA)). At time 2.5 hours one of each triplicate was induced with 1 mM IPTG. No growth change was observed.