Part:BBa_K1088027

Dxs from B. subtilis with lac promoter and lacI (IPTG inducible)

The part consist of the dxs gene derived from B. subtilis under the control of the lac promoter and has a strong RBS.

To repress expression from the lac promoter, the lacI gene under a constitutive promoter, with a strong RBS and a efficient terminator is placed counter-clockwise to the reporter fusion. The repression can then be relieved with addition of IPTG, which binds and inhibits the function of LacI.

The purpose of the brick is to increase the flow through the MEP pathway upon addition of IPTG.

A similar part (BBa_K1088026) with a GFP fused to Dxs, was used to prove that the part is IPTG inducible. See reporter fusion for details.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2460
  Illegal EcoRI site found at 3117
  Illegal PstI site found at 2518
  Illegal PstI site found at 2964
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2460
  Illegal EcoRI site found at 3117
  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 2518
  Illegal PstI site found at 2964
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2460
  Illegal EcoRI site found at 3117
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2460
  Illegal EcoRI site found at 3117
  Illegal PstI site found at 2518
  Illegal PstI site found at 2964
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2460
  Illegal EcoRI site found at 3117
  Illegal PstI site found at 2518
  Illegal PstI site found at 2964
  Illegal NgoMIV site found at 2417
  Illegal AgeI site found at 2310
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2259
  Illegal SapI.rc site found at 2958