Device

Part:BBa_K1065309:Design

Designed by: Fabio Digiacomo   Group: iGEM13_UNITN-Trento   (2013-09-18)
Revision as of 12:15, 18 September 2013 by Fabius924 (Talk | contribs) (→‎Source)


light regulated circuit producing amilGFP+EFE at dark


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 842
    Illegal NgoMIV site found at 932
    Illegal NgoMIV site found at 950
    Illegal NgoMIV site found at 1462
    Illegal NgoMIV site found at 1755
    Illegal NgoMIV site found at 1849
    Illegal AgeI site found at 484
    Illegal AgeI site found at 1630
    Illegal AgeI site found at 4060
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1519
    Illegal BsaI.rc site found at 383


Design Notes

this part doesn't present any inverter cassette, so transcription is activated at dark.

Source

the part sources are: Bba_K1065302 (our improved part) and BBa_K1065002 (EFE+terminators), both submitted in the registry by our team.

References