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Part:BBa_J32006:Design

Designed by: Austen Heinz   Group: iGEM06_Duke   (2006-07-11)
Revision as of 03:29, 12 July 2006 by Ajh20 (Talk | contribs)

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Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1440
    Illegal PstI site found at 1566
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 364
    Illegal PstI site found at 1440
    Illegal PstI site found at 1566
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 638
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1440
    Illegal PstI site found at 1566
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1440
    Illegal PstI site found at 1566
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 1274
    Illegal AgeI site found at 1049
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 119
    Illegal BsaI.rc site found at 330
    Illegal SapI.rc site found at 530


Design Notes

Fusion Protein


Source

The signal sequence PelB and S*Tag sequence came from Novagen. The antibody sequence is for cAb-CEA5 nanobody from Ablynx. The C-terminus of Neisseria gonorrhoeae IgA Protease NCBI accession number AE004969

References