Part:BBa_K1062003

Guide RNA (gRNA) target for XylE (end of gene)

This part is a guide RNA (gRNA) that targets the end of the XylE gRNA, when bound to Cas9.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

In both of our projects, we used gRNAs and dCas9 as: 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project However, the gRNA for XylE is not used in the conjugation project since we did not use any of the pigments in this project. 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.

Conjugation Project

The XylE (middle) gRNA was not used in the Conjugation Project

Synthetic Circuit