Device
Part:BBa_K1150040
Designed by: Ilona Blank Group: iGEM13_Freiburg (2013-09-17)
uniCAS RNAimer to Bla Target 2
RNaimer (Bla target 2) | |
---|---|
Function | Targeting VEGF2 with Cas9 |
Use in | Mammalian cells |
RFC standard | RFC 25 |
Backbone | pSB1C3 |
Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Usage and Biology
This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered dCas9 protein to the Bla locus target 2 . This RNaimer is bulid of the uniCAS RNAimer and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 224
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216
[edit]
Categories
Parameters
None |