RBS
rRBS-2

Part:BBa_K1017202:Design

Designed by: Wen-Hui Cheng   Group: iGEM13_NCTU_Formosa   (2013-09-12)
Revision as of 13:43, 26 September 2013 by AggieTsai (Talk | contribs) (→‎References)

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Regulation RBS-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The purpose of the rRBS-2 is to work with sRNA-2. When the sRNA-2 bind on the shine-dalgarno sequence of rRBS-2, it would block the translation by compete between ribosome and sRNA-2.


Source

synthesis

References

Torsten Waldminghaus, Nadja Heidrich, Sabine Brantl and Franz Narberhaus .(2012). Engineering Artificial Small RNAs for Conditional Gene Silencing in Escherichia coli ,1: 6–13