Reporter

Part:BBa_K1041001:Experience

Designed by: NRP UEA   Group: iGEM13_NRP-UEA-Norwich   (2013-08-08)
Revision as of 11:14, 17 September 2013 by Holusac (Talk | contribs) (→‎Team NRP-UEA_Norwich 2013)


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Team NRP-UEA_Norwich 2013

Team NRP-UEA_Norwich 2013 designed this part to contain the promoter sequence AntG, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene. This facilitated cloning of the biobrick Bba_K1041002

Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.

Sequencing

The biobrick was sent off to a company for sequencing.

K1041000 sequencing data part 1
K1041000 sequencing data part 2
K1041000 sequencing data part 3










BLAST Analysis

The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence fig.2

User Reviews

UNIQ6e7148f87daed5a7-partinfo-00000000-QINU UNIQ6e7148f87daed5a7-partinfo-00000001-QINU

User Reviews

UNIQ6e7148f87daed5a7-partinfo-00000002-QINU UNIQ6e7148f87daed5a7-partinfo-00000003-QINU