Part:BBa_K1019005

ompA: outer membrane protein 3a

OmpA is believed to be a porin, a class of nonspecific diffusion channel that allows small solutes to pass through the outer membrane. It is also believed to stabilize cellular shape.

Contrary to the theme of our other parts being used to increase biofilm formation, ompA has actually been shown to decrease biofilm formation by repressing the expression of cellulose, a biofilm component.

OmpA deletions display sticky colony morphology as a result of excess cellulose being produced.

Extensive information regarding ompA can be found at [http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10669 EcoCyc]

References

Sugawara, E., & Nikaido, H. (1992). Pore-forming activity of OmpA protein of Escherichia coli. Journal of Biological Chemistry, 267(4), 2507-2511.

Barrios, A. F. G., Zuo, R., Ren, D., & Wood, T. K. (2006). Hha, YbaJ, and OmpA regulate Escherichia coli K12 biofilm formation and conjugation plasmids abolish motility. Biotechnology and bioengineering, 93(1), 188-200.

Ma, Q., & Wood, T. K. (2009). OmpA influences Escherichia coli biofilm formation by repressing cellulose production through the CpxRA two‐component system. Environmental microbiology, 11(10), 2735-2746.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 881
  Illegal BamHI site found at 744
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]