Reporter

Part:BBa_J04450

Designed by: Tamar Odle   Group: iGEM2005   (2005-06-09)
Revision as of 15:38, 23 July 2009 by Meagan (Talk | contribs)


RFP Coding Device

LacI
R0010
B0034		mRFP1
E1010
B0015

The colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong.

  • LacI sensitive
  • CAP sensitive

This part is commonly used, but can fail if the system contains LacI or CAP protein.


Pictures

  • BBa_J04450 visualized under non-UV lightbox

  • BBa_J04450 visualized under 254nm wavelength UV lightbox

  • BBa_J04450 Colonies

[edit]
Categories
//cds/reporter/chromoprotein
//classic/reporter/pret
//function/reporter/color
//function/reporter/pigment
Parameters
emissionRFP
excitation
tagNone