Composite

Part:BBa_K1149006

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-13)
Revision as of 19:17, 15 September 2013 by Margarita K (Talk | contribs)

pelB-pulA-flag expression

Expression of a plastic degradation enzyme from Pseudomonas fluorescens under arabinose inducible promoter. The protein in codon optimized for expression in ecoli, is flag tagged and has an Nterminal pelB tag for secretion. Encodes polyurethane esterase, break down ester bonds in polyurethane.

PUR degradation by esterase: PUR_esterase_theoretical_pathway_2.jpg


references:

[http://www.sciencedirect.com/science/article/pii/S0964830598000687 Cloning and expression in Escherichia coli of a polyurethane-degrading enzyme from Pseudomonas fluorescens]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 804
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1099
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/degradation
Parameters
None