Part:BBa_K1159105

Mature Nuclease NucA from Staphylococcus aureus (Thermonuclease) in RFC[25]

Thermonuclease is a endo-exonuclease from Staphylococcus aureus that degrades dsDNA, ssDNA, dsRNA and ssRNA. This part is coding the mature form of the nuclease, also called NucA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein)
ATGGCCGGCGCAACTTCA ... TCAGGTCAAACCGGT
ORF from nucleotide position -8 to 453 (excluding stop-codon)

Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

1
101

MAGATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGK
MVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQTG*

Sequence features: (with their position in the amino acid sequence, see the list of supported features)

None of the supported features appeared in the sequence

Amino acid composition:

Ala (A)	15 (9.7%)
Arg (R)	5 (3.2%)
Asn (N)	6 (3.9%)
Asp (D)	8 (5.2%)

Cys (C)	0 (0.0%)
Gln (Q)	6 (3.9%)
Glu (E)	12 (7.8%)
Gly (G)	12 (7.8%)

His (H)	4 (2.6%)
Ile (I)	5 (3.2%)
Leu (L)	11 (7.1%)
Lys (K)	23 (14.9%)

Met (M)	5 (3.2%)
Phe (F)	3 (1.9%)
Pro (P)	6 (3.9%)
Ser (S)	5 (3.2%)

Thr (T)	11 (7.1%)
Trp (W)	1 (0.6%)
Tyr (Y)	7 (4.5%)
Val (V)	9 (5.8%)

Amino acid counting

	Total number:	154
	Positively charged (Arg+Lys):	28 (18.2%)
	Negatively charged (Asp+Glu):	20 (13.0%)
	Aromatic (Phe+His+Try+Tyr):	15 (9.7%)

Biochemical parameters

	Atomic composition:	C₇₆₃H₁₂₃₁N₂₁₃O₂₃₀S₅
	Molecular mass [Da]:	17228.8
	Theoretical pI:	9.48
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	15930 / 15930 (all Cys red/ox)

Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges

Codon usage

	Organism:	E. coli	B. subtilis	S. cerevisiae	A. thaliana	P. patens	Mammals
	Codon quality (CAI):	good (0.79)	excellent (0.83)	excellent (0.82)	excellent (0.81)	good (0.72)	acceptable (0.60)

Alignments (obtained from PredictProtein.org)

	SwissProt:	P00644 (99% identity on 150 AAs), Q5HHM4 (98% identity on 150 AAs), Q6GB41 (98% identity on 150 AAs), Q6GIK1 (98% identity on 150 AAs), Q7A6P2 (98% identity on 150 AAs), Q8NXI6 (98% identity on 150 AAs), Q99VJ0 (98% identity on 150 AAs)
	TrEML:	A3QSR2 (98% identity on 140 AAs), A3QSR3 (98% identity on 140 AAs), A3QSR6 (98% identity on 140 AAs), A5A513 (98% identity on 148 AAs), A5A520 (98% identity on 148 AAs), A5A523 (98% identity on 148 AAs), A5IQZ5 (98% identity on 150 AAs), A6QFA0 (98% identity on 150 AAs), A6TZS0 (98% identity on 150 AAs), A7WZZ3 (98% identity on 150 AAs)
	PDB:	1ey0 (100% identity on 136 AAs), 1eyd (100% identity on 136 AAs), 1nsn (100% identity on 138 AAs), 1snc (100% identity on 135 AAs), 1sta (100% identity on 135 AAs), 1stb (100% identity on 136 AAs), 1stg (100% identity on 136 AAs), 1sth (100% identity on 136 AAs), 1stn (100% identity on 136 AAs), 1sty (100% identity on 136 AAs)

Predictions (obtained from PredictProtein.org)

Subcellular Localization (reliability in brackets)

	Archaea:	cytosol (100%)
	Bacteria:	secreted (55%)
	Eukarya:	cytosol (24%)

Gene Ontology (reliability in brackets)

	Molecular Function Ontology:	-
	Biological Process Ontology:	GO:0030541 (28%)

Predicted features:

	Disulfid bridges:	-
	Transmembrane helices:	-

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