Generator

Part:BBa_K1076004:Design

Designed by: Carlos Gustavo Nunes   Group: iGEM13_Manaus_Amazonas-Brazil   (2013-09-13)
Revision as of 00:16, 20 September 2013 by Lais (Talk | contribs) (→‎Design Notes)


FadD, Long-chain-fatty-acid-CoA ligase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1883
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1934
    Illegal NgoMIV site found at 1944
    Illegal AgeI site found at 1462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 415


Design Notes

The idea is to compose a part with parts from the Registry powerful promoter, compatible RBS and terminators. The gene was amplified within EcoR1 and Pst1 restriction sites in order to put into pSB1C3. Further subclonning and expression should be done within pSB3C5-152001.

Source

Shewanella putrefaciens genomic sequence

References