Reporter

Part:BBa_K1087015:Experience

Designed by: Tong Xu   Group: iGEM13_SCU_China   (2013-09-13)
Revision as of 03:33, 28 September 2013 by Nianhui (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K1087015

User Reviews

UNIQ9d94b0745db28145-partinfo-00000000-QINU UNIQ9d94b0745db28145-partinfo-00000001-QINU

http://2013.igem.org/Team:SCU_China/Project

We transformed BBa_K1087015, BBa_K1087021into E.coli DH5α,respectively. And we use Ptet+mRFP1(BBa_I13521) as positive control, irrelevant BBa_J61046 with no fluorescence gene as negative control. We used fluorescence microscope to observe the fluorescence at 16h, and we also used Thermo varioskan flash to quantitatively measured the fluorescence intensity at 16h,18h, and 19h, respectively.

According to the data,the ribolock BBa_K1087004 works well, and its fluorescence intensity was only 2.6531% compared to positive control even at 19h, while that of lock & key (BBa_K1087021) was 91.7138% at 19h. The results suggest the key BBa_K145215 can hugely improve the expression level of the lock BBa_K1087004.