Measurement

Part:BBa_K1100011

Designed by: YingQi Lou   Group: iGEM13_Fudan   (2013-09-13)
Revision as of 16:27, 27 September 2013 by Cheese7 (Talk | contribs)

B0015 terminator efficiency estimation device (with Csy4 system)

In this device, we test the terminator efficiency in the consideration that the terminator is influenced by the downstream sequence. The csy4 cleavage site is inserted just after the terminator(BBa_B0015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 164


[edit]
Categories
Parameters
absB0034
biology
chassisE.coli
controlaTc, tetracyline
directionForward
efficiency1
emission510nm
excitation485nm
negative_regulators1
o_h
o_l
positive_regulators
proteinsfGFP