Regulatory

Part:BBa_K1078000:Design

Designed by: Edgar Andres Ochoa Cruz   Group: iGEM13_USP-Brazil   (2013-09-12)
Revision as of 18:37, 27 September 2013 by Andresochoa (Talk | contribs) (→‎Design Notes)

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Mxr1 (methanol expression regulator 1) modified


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 240
    Illegal XhoI site found at 137
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 430
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 179
    Illegal SapI.rc site found at 635


Design Notes

After synthesis, in order to build our sensor the part was introduced in pPIC9K plasmid to allow its genomic recombination into the Pichia pastoris genome, substituting the original Mxr1 found in the yeast. The sequenced was modified to remove restriction sites, making it compatible to RFC10

Source

The part was obtained by synthesis.

References

The modified Mxr1 sequence was found in Parua PK, Ryan PM, Trang K, Young ET: Pichia pastoris 14-3-3 regulates transcriptional activity of the methanol inducible transcription factor Mxr1 by direct interaction. Mol Microbiol 2012, 85:282-298.