Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Translational_Unit

Part:BBa_K1017602

Designed by: Wen-Hui Cheng   Group: iGEM13_NCTU_Formosa   (2013-09-12)
Revision as of 18:07, 17 September 2013 by Mike821005 (Talk | contribs)

37℃ RBS + mGFP

This biobrick is comprised of 37 degrees Celsius RBS and mGFP. 37℃ RBS, a RBS which on/off is controlled by temperature. mRFP is a red fluorescent protein. When the temperature is higher than 37℃, 37℃ RBS functions normally and the downstream gene can be expressed. In contrast with high temperature,37℃ RBS forms a hairpin structure that block the ribosome binding when lower 37℃. Thus, the downstream gene, mGFP, is not able to translate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 702


[edit]
Categories
Parameters
None