Reporter

Part:BBa_K1155003:Experience

Designed by: Sylvie Lautru, Anas, Zhou, Abdou   Group: iGEM13_Paris_Saclay   (2013-09-10)
Revision as of 22:22, 4 October 2013 by SolenneParisSaclay (Talk | contribs) (→‎Applications of BBa_K1155003)


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Applications of BBa_K1155003

We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3

BBa_K1155000 is a promoteur Pndh* repressed in anaerobic condition by binding of the FNR regulator.(see BBa_K1155000 for more information)

What we expected :

+ O2 : FNR inactivated : Pndh* activated --> amilCP expression --> purple color

- O2 : FNR activated : Pndh* repressed --> amilCP not expressed --> no color

What we observed :

+ O2 : --> purple color

- O2 : --> no color

[photo]

These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression.

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