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Part:BBa_K1139021:Experience

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-09-09)
Revision as of 09:35, 26 September 2013 by Keso57 (Talk | contribs)

Plux-M13-Plac-GFP on pSB3

We confirmed whether the release of M13 phage particle depends on the induction of g2p expression. We inserted lux promoter (activated by LuxR-3OC6HSL complex) upstream of g2p, as an inducible promoter.

Fig. 1. Flow chart of this experiment.

Prepare E. coli surrounded by a red circle. We add inducer (AHL) to the E. coli. Phage is released by induction of AHL. Spin the culture of the E. coli. Decant the supernatant of the culture including phages to soft agar ( the agar which is easy to melt ). Add lawn (E. coli containing pSB6A1-Ptet-luxR ) to the soft agar. Mix them in soft agar. Then, decant the soft agar to YT plate.

1. Materials and Method

2. Result

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