Composite

Part:BBa_K1140002

Designed by: Janssel Reyes del Castillo   Group: iGEM13_UANL_Mty-Mexico   (2013-09-08)
Revision as of 01:07, 19 September 2013 by GilbertoRdz (Talk | contribs)

pLac + 32 oC RNA thermometer + GFP(LVA)

The gene is under the regulation of a pLac promoter and a thermolabile ribosome binding site (RBS) that should allow for translation only at temperatures above 32°C. At the 3’ end of the gene dubbed “GFP-LVA” are two consecutive transcription termination regions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 797


[edit]
Categories
Parameters
directionForward
functionReporter
proteinGFP
rbs32 oC riboswitch
tagLVA